Journal of Nutraceuticals and Health

ABSTRACT

Background: To determine the osteoclastogenesis inhibitory effect and inside mechanisms of a curcuminoids-rich extract (CRE) and three water-soluble CREs, namely CRE-SD (CRE in solid dispersion form), CRE-Bin (CRE binary complex with hydroxypropyl-Î-cyclodextrin), and CRE-Ter (CRE ternary complex with hydroxypropyl-Î-cyclodextrin and polyvinylpyrrolidone K30), as well as curcumin (Cu), bisdemethoxycurcumin (Bis), and demethoxycurcumin (De).

Methods: TRAP (tartrate-resistant acid phosphatase) and acid phosphatase assays were used to confirm osteoclastogenesis. MTT [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole] and LDH (lactate dehydrogenase) assays were performed to evaluate cell viability and cell cytotoxicity, respectively. Mechanisms of action and signaling pathways were explored using Western blot analysis. Bone degradation was measured using a bone pit and fluorescein assays.

KEYWORDS

1. Curcumin
2. Curcuminoid
3. Inclusion complex
4. Solid dispersion
5. Ternary complex